Figure 2. Comparison of the experimental and predicted pI values for the set of 7390 peptides [5]. This set was obtained during analysis of peptides after isoelectric focusing and the experimental values were determined with accuracy ± 0.15 pH units, (or the "step" of 0.3 pH units). On the left side are shown values predicted by the widely used "Compute pI / Mw" service (https://web.expasy.org/compute_pi/). On the right side, the predictions by the new pIPredict 2 scale are shown.