Table 2. Protocol of SPR registration of protein – protein interactions. Executed commands were derived from the queue window Biacore 3000 Control Software.
|
Time, s |
Info |
|
0 |
Timestamp: 14-May-18 14:00:24 |
|
0 |
Set Temperature: 25.0°C |
|
0 |
Temperature: 24.99°C |
|
0 |
Detection Mode: 1,2 |
|
0 |
Rack 1 (Left): THERMO_C |
|
0 |
Rack 2 (Right): THERMO_A |
|
0 |
Rack R (Reagent): Reag_A |
|
0 |
Flow Cell: 1-2 |
|
0 |
Flow: 10 μl/min |
|
0 |
Data Collection: 1 Hz |
|
939.4 |
Transfer: Start 450 μl B->R2A1 |
|
1023.5 |
Transfer: Ready |
|
1587 |
Start QUICKINJECT |
|
1619.9 |
Inject: Start Fc 1-2 R2A1 |
|
1919.7 |
Inject: Ready 50 μl |
|
1955.6 |
End QUICKINJECT |
|
2147.1 |
Transfer: Start 450 μl B->R2A2 |
|
2231.2 |
Transfer: Ready |
|
2690.9 |
Start QUICKINJECT |
|
2721.1 |
Flowfill: Start |
|
2729.1 |
Flowfill: Ready |
|
2761.3 |
Inject: Start Fc 1-2 R2A1 |
|
2790.8 |
Inject: Ready 5 μl |
|
2824.1 |
End QUICKINJECT |
|
2938.4 |
Start QUICKINJECT |
|
2971.7 |
Inject: Start Fc 1-2 R2B1 |
|
3271.6 |
Inject: Ready 50 μl |
|
3307.4 |
End QUICKINJECT |
|
3347 |
Start QUICKINJECT |
|
3380 |
Inject: Start Fc 1-2 R2C1 |
|
3559.6 |
Inject: Ready 30 μl |
|
3594.4 |
End QUICKINJECT |
|
3624.6 |
Start QUICKINJECT |
|
3655.7 |
Inject: Start Fc 1-2 R2A1 |
|
3685.2 |
Inject: Ready 5 μl |
|
3718.1 |
End QUICKINJECT |
|
4149.3 |
Transfer: Start 450 μl B->R2A1 |
|
4233.6 |
Transfer: Ready |
|
4382.7 |
Start QUICKINJECT |
|
4416 |
Inject: Start Fc 1-2 R2B1 |
|
4715.8 |
Inject: Ready 50 μl |
|
4751.4 |
End QUICKINJECT |
|
4752.4 |
Start QUICKINJECT |
|
4784.7 |
Inject: Start Fc 1-2 R2C1 |
|
4964.4 |
Inject: Ready 30 μl |
|
4998.6 |
End QUICKINJECT |
|
5002.5 |
Start QUICKINJECT |
|
5033.5 |
Inject: Start Fc 1-2 R2A1 |
|
5063 |
Inject: Ready 5 μl |
|
5095.5 |
End QUICKINJECT |
|
5412.1 |
Start QUICKINJECT |
|
5442.2 |
Flowfill: Start |
|
5450 |
Flowfill: Ready |
|
5482.3 |
Inject: Start Fc 1-2 R2A1 |
|
5511.8 |
Inject: Ready 5 μl |
|
5544.7 |
End QUICKINJECT |
|
6391.5 |
Temperature: 25.0°C |