Table 1. Characteristics of synthesized peptide p-nitroanilide thrombin substrates.
Substrate |
Amount, mg |
Yield, % theoretica |
Purity, % |
[M+H]+, m/e calc. |
[M+H]+, m/e experim. |
Bz-FVR-pNA |
64 |
40 |
>95 |
645.3 |
645.4 |
Bz-VPR-pNA |
58 |
41 |
>95 |
595.3 |
595.4 |
Bz-GPR-pNA |
54 |
39 |
>95 |
553.3 |
553.3 |
Bz-LTPR-pNA |
53 |
30 |
>95 |
710.4 |
710.4 |
Note. а Calculation was based on the amount of p-phenylene diamine residues on the resin.