Table 1. Characteristics of synthesized peptide p-nitroanilide thrombin substrates.

Substrate

Amount, mg

Yield, % theoretica

Purity, %

[M+H]+, m/e calc.

[M+H]+, m/e experim.

Bz-FVR-pNA

64

40

>95

645.3

645.4

Bz-VPR-pNA

58

41

>95

595.3

595.4

Bz-GPR-pNA

54

39

>95

553.3

553.3

Bz-LTPR-pNA

53

30

>95

710.4

710.4

Note. а Calculation was based on the amount of p-phenylene diamine residues on the resin.