Table 2. List of Nt-acetylated peptides of proteins identified in control and anembryonic chorionic villi (CVS) via PTM-sensitive MS/MS search (Mascot searching with taking into account possible protein Nt-acetylation).

 

Accession

Description

Modified peptide

Control CVS

Anembryonic CVS

1

AL1A1_HUMAN

Retinal dehydrogenase 1

M. SSSGTPDLPVLLTDLK.I + Acetyl (Protein N-term)

+

+

2

AL9A1_HUMAN

4-trimethylaminobutyraldehyde dehydrogenase

M. STGTFVVSQPLNYR.G + Acetyl (Protein N-term)

+

+

3

CNDP2_HUMAN

Cytosolic non-specific dipeptidase

M. AALTTLFK.Y + Acetyl (Protein N-term)

nd

+

4

KAP2_HUMAN

cAMP-dependent protein kinase type II-alpha regulatory subunit

M. SHIQIPPGLTELLQGYTVEVLR.Q + Acetyl (Protein N-term)

+

+

5

G6PD_HUMAN

Glucose-6-phosphate 1-dehydrogenase

M. AEQVALSR.T + Acetyl (Protein N-term)

+

+

6

GSHB_HUMAN

Glutathione synthetase

M. ATNWGSLLQDKQQLEELAR.Q + Acetyl (Protein N-term)

+

nd

7

SEPT6_HUMAN

Septin-6

M. AATDIAR.Q + Acetyl (Protein N-term)

+

+

8

SEP11_HUMAN

Septin-11

M. AVAVGRPSNEELR.N + Acetyl (Protein N-term)

+

+

9

RHG01_HUMAN

Rho GTPase-activating protein 1

-. MDPLSELQDDLTLDDTSEALNQLK.L + Acetyl (Protein N-term)

+

+

10

PUR8_HUMAN

Adenylosuccinate lyase

M. AAGGDHGSPDSYR.S + Acetyl (Protein N-term)

nd

+

11

PSMD5_HUMAN

26S proteasome non-ATPase regulatory subunit 5

M. AALTTLFK.Y + Acetyl (Protein N-term)

+

+

12

TCPB_HUMAN

T-complex protein 1 subunit beta

M. ASLSLAPVNIFK.A + Acetyl (Protein N-term)

+

nd

13

ACOC_HUMAN

Cytoplasmic aconitate hydratase

M. SNPFAHLAEPLDPVQPGKK.F + Acetyl (Protein N-term)

+

+

14

DX39B_HUMAN

Spliceosome RNA helicase DDX39B

M. AENDVDNELLDYEDDEVETAAGGDGAEAPAKK.D + Acetyl (Protein N-term)

+

nd

15

TKT_HUMAN

Transketolase

-. MESYHKPDQQK.L + Acetyl (Protein N-term)

+

nd

Note. nd – not detectable.