Table 1. Primers used in PCR .
|
Primer |
Nucleotide sequence (5' → 3') |
| Mat-RNLS-for |
gagctc ggatcc ga aatggaagcg ctgctgaggaggcag |
|
Mat-RNLS-rev(+stop) |
tcgtg ctcgag ctaaatataattctttaaagcttccag |
|
Mat-RNLS-rev(-stop) |
tcgtg ctcgag aatataattctttaaagcttccag |
Note. BamHI, XhoI and Kozak restriction sites are shown in bold italics.