Table 1. Superoxide generating activity of the studied compounds.
|
Compounds |
Concentration, mM |
Velocity, (∆ optical density, E560 nm / min) |
Примечания |
|
NADPH |
1.5 |
0.0163±0.0026 |
|
|
NADP |
1.5 |
0.0071±0.0011 |
|
|
NADH |
1.5 |
0.0070±0.0010 |
|
|
NAD |
1.5 |
No kinetics within 10 min |
diformazan detected after 4 h |
|
NADH |
1.0 |
0.0046±0.0002 |
see also fig. 1-B, curve 1 |
|
NADH + SOD |
1.0 |
0,0013±0.0002 |
SOD, 0.92 mkg/ml, see also fig. 1-B, curve 2 |
|
Adenosine |
1.5 |
No kinetics within 10min |
no diformazan after 19 h |
|
АDP |
1.5 |
No kinetics within 10 min |
no diformazan after 19 h |
|
АТP |
1.5 |
No kinetics within 10 min |
no diformazan after 19 h |
Note: Reaction conditions: 0.2 M carbonate buffer, pH 10.8, 0.075 mm HCT, registration 10 min. The celocity was calculated for the first minute from the start of registration, explanations in "Methods".