Figure 1 The representative kinetic curves of a cleavage of FQ-reporters by Cas13a nuclease: the fluorescence intensity (in arbitrary units, a.u.) in the absence (curves 1, 2, and 3) and the presence (curves 4, 5, and 6) of miRNAs is shown at every measurement time point. Cas13a in the complex with gRNA targeting miR-145 (curves 1 and 4), miR-218 (curves 2 and 5), and miR-34a (curves 3 and 6). 30 ng of miRNA per reaction.