Figure 4. The impact of background RNA on a collateral activity of gRNA/Cas13a complex targeting miR-34a (A) or miR-218 (B). The difference between fluorescence in the presence (F) and the absence (F0) of RNA at 1 h incubation time for various combinations of miRNAs and total RNA. 1 – miRNA only, 2, 3, and 6 – miRNA in the presence of E. coli total RNA, HepG2 total RNA, and small RNA from HepG2 cells, respectively. 4 and 5 – only total RNA from E. coli and HepG2 cells, respectively. The amount of miRNA – 3 ng per reaction tube, total and small RNAs – 100 ng per reaction tube.