Biomedical Chemistry: Research and Methods, 2025, 8(4), e00265

ГЕТЕРОЛОГИЧНАЯ ЭКСПРЕССИЯ ГЕНОВ РЕКОМБИНАНТНЫХ L-АСПАРАГИНАЗ
(Посвящается памяти профессора Н.Н. Соколова, внесшего суущественный вклад в исследования L-аспарагиназ)

М.В. Покровская*, С.С. Александрова, В.С. Покровский, Н.Д. Добрякова, А.Н. Шишпаренок,
Ю.В. Гладилина, Д.Д. Жданов

Научно-исследовательский институт биомедицинской химии им. В.Н. Ореховича, 119121, Москва, ул. Погодинская, 10; *e-mail: Ivan1190@yandex.ru

Ключевые слова: L-аспарагиназа, оптимизация экспрессии, гетерологичная экспрессия, рациональный и компьютерный дизайн, рекомбинантные гены

DOI:10.18097/BMCRM00265

L-аспарагиназa (КФ 3.5.1.1.) — фермент с самым высоким уровнем мирового производства, используемый при лечении онкологических заболеваний, а также в пищевой промышленности. Для производства многих целевых белков используются различные системы экспрессии – от бесклеточных до гиперпродуктивных клеток растений, насекомых, бактерий и млекопитающих. В данном обзоре предпринята попытка обобщить общирные данные по экспрессии гетерологичных генов и технологии производства рекомбинантных L-аспарагиназ.

Рисунок 1. Схематическое представление механизма действия L-аспарагиназ. Описание в тексте.

Рисунок 2. Стратегия гетерологичной экспрессии L-аспарагиназ. Описание в тексте.

Рисунок 3. Классификация аспарагиназ (адаптировано из [31]).Описание в тексте.

Рисунок 4. Lac-оперон E. coli.Описание в тексте.

Рисунок 5. Схема строения плазмидного вектора. Описание в тексте.

Рисунок 6. Инициация трансляции у бактерий. Описание в тексте.

ФИНАНСИРОВАНИЕ

Работа выполнена в рамках Программы фундаментальных научных исследований в Российской Федерации на долгосрочный период (2021-2030 годы) (№ 122022800499-5).

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