Biomedical Chemistry: Research and Methods 2025, 8(4), e00265

HETEROLOGOUS EXPRESSION OF RECOMBINANT L-ASPARAGINASE GENES
(Dedicated to late Professor N.N. Sokolov, who made a significant contribution in the field of L-asparaginase)

M.V. Pokrovskaya *, S.S. Alexandrova, V.S. Pokrovsky, N.D. Dobryakova, A.N. Shishparenok,
Yu.V. Gladilina Ю.В., D.D. Zhdanov

Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121; *e-mail: Ivan1190@yandex.ru

Keywords:L-asparaginase, expression optimization, heterologous expression, rational and computer-aided design, recombinant genes

DOI:10.18097/BMCRM00265

The whole version of this paper is available in Russian.

L-asparaginase (EC 3.5.1.1.) is the enzyme with the highest level of global production and is used in the treatment of cancer and in the food industry. Different expression systems are used for the production of many target proteins, ranging from cell-free to hyperproductive plant, insect, bacterial and mammalian cells. This review attempts to bring together the available literature data on heterologous gene expression and technology for the production of recombinant L-asparaginases.
Figure 1. Schematic representation of the mechanism of action of L-asparaginases.

Figure 2. Strategy for heterologous expression of L-asparaginases. Description in text.

Figure 3. Classification of asparaginases (adapted from [31]). Description in text.

Figure 4. Lac operon of E. coli. Description in text.

Figure 5. Plasmid vector structure diagram. Description in text.

Figure 6. Translation initiation in bacteria. Description in text.

FUNDING

The work was carried out within the framework of the Program of Fundamental Scientific Research in the Russian Federation for the long-term period (2021-2030) (No. 122022800499-5).

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