Biomedical Chemistry: Research and Methods, 2018, 1(3), e00033
The 40th Anniversary of the Institute of Physiologically Active Compounds of the Russian Academy of Sciences

The Effect of New Synthetic Analogs of Semax Peptide Preparation on AMPA-Receptors
in Purkinje Neurons of Cerebellar Rats

V.V. Grigoriev1, V.L. Zamoyski1, E.V. Bovina1*, L.A. Andreeva2, N.F. Myasoedov2

1Institute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severny proezd, Moscow region, Chernogolovka, 142432 Russia;*e-mail: bovina_e@ipac.ac.ru
2Institute of Molecular Genetics of the Russian Academy of Sciences, 2, Academician I.G. Kurchatov’s square, Moscow, 123182 Russia

Key words: peptide analogues of Semax; AMPA receptors; Purkinje neurons

DOI: 10.18097/BMCRM00034

The whole version of this paper is available in Russian.

New peptides analogues of  Semax can potentiate AMPA receptors induced currents at lower concentrations (10–11–10–8 M) than those of Semax. This ability suggests that they may have cognitive-stimulating properties.
Figure 1. The effect of peptides nos. 1, 2 and 3 on the amplitude of AMPA-receptor induced currents in Purkinje neurons of the cerebellum of rats.
lgC is the decimal logarithm of concentrations of compounds, M; % is the amplitude of kainate-induced currents in the presence of compounds to the control taken as 100%.

Figure 2. The effect of peptides KKRRPGP (4), MIT-1 (5) and MIT-2 (6) on the amplitude of AMPA-receptor currents in Purkinje neurons of the cerebellum of rats.
lgC is the decimal logarithm of concentrations of compounds, M; % is the amplitude of kainate-induced currents in the presence of compounds to the control taken as 100%.

ACKNOWLEDGEMENTS

The study was conducted in accordance to the Research Topic No. 48.8 «The search and determination of the mechanisms of neuroprotectors and stimulators of cognitive functions» in the framework of the State Task for the Institute of Physiologically Active Compounds of the Russian Academy of Sciences (Topic: No: 0090-2017-0019).

The equipment of the Centre of collective usage of IPAS RAS was used in the work (Agreement No. 14.621.21.0008, work identifier RFMEFI62114X0008).

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