Biomedical Chemistry: Research and Methods, 2018, 1(3), e00038
The 40th Anniversary of the Institute of Physiologically Active Compounds of the Russian Academy of Sciences

Assessment of the Adaptation Capacities of the Body Based on the Determination of the Natural Antibodies to the Endogenous Bioregulators

M.A. Myagkova*, S.N.Petrochenko, A.I. Levashova

Institute of Physiologically Active Compounds of the Russian Academy of Sciences, 1 Severny proezd, Moscow region, Chernogolovka, 142432 Russia,*e-mail: m.a.myagkova@gmail.com

Key words: athlete's adaptation potential; gamma-aminobutyric acid; glutamic acid; diagnostic markers; enzyme immunoassay; training load

DOI: 10.18097/BMCRM00038

The whole version of this paper is available in Russian.

A comparative analysis of natural antibodies to bioregulators of the excitation and inhibition nervous system (glutamate and GABA) was performed in serum of people with different types of physical activity. Immunological monitoring was done in three stages: on day 1 (stage 1), on day 14 (stage 2) and on day 28 of examination (stage 3). The study included individuals who were intensively engaged in certain kinds of sports (Greco-Roman wrestling, hockey) and volunteers, who normally practiced a set of health physical exercises. A significant increase in the level of natural antibodies to GABA and glutamate was found with an increase in the duration of exercise in the training process compared with the control group. For individuals involved in various sports activities, the relationship between the intensity of the loads and the immunological parameters was established. Practical recommendation for the application of the developed method for an estimation of adaptation possibilities of the organism at changing physical loadings is given.

Figure 1. The number of athletes (in %) with altered levels of antibodies to GABA and glutamate at rest and after physical load in the group of hockey players.

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Table 1. ELISA conditions for the detection of natural antibodies to GABA and glutamic acid in analyzed serum blood samples.

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Table 2. Levels of natural antibodies (OD450 in ELISA (М±m)) in serum blood samples of examined individuals at different stage of monitoring.

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Table 3. Levels of natural antibodies (OD450 in ELISA (М±m)) in blood serum in groups with different duration of a sport career.

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Table 4. Levels of natural antibodies (OD450 in ELISA (М±m)) in blood serum at rest period and after physical loading

ACKNOWLEDGEMENTS

The work was carried out within the framework of the Program of Fundamental Scientific Research of the State Academies of Sciences for 2013-2020 (State Registration No. 01200952666).

The authors thank the professor Spasskii A.A., general Secretary of the Russian Scientific Medical Society of Physicians for the organization of the work with a group of surveyed athletes.

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