Transcriptional Analysis of HeLa Cells - Producers of the Recombinant Peptidoglycan Recognition Protein PGLYRP1 at Different Stages of the Chlamydia Trachomatis Infection Development
Federal Research and Clinical Center of Physical-Chemical Medicine,
1a Malaya Pirogovskaya str., Moscow, 119435 Russia; *e-mail: email@example.com
Keywords:Chlamydia trachomatis; PGLYRP; recombinant proteins; transcriptomics
Human peptidoglycan recognition proteins (PGLYRPs) are the components of innate immunity that exhibit antibacterial activity. In this study a cell line secreting recombinant PGLYRP1 into a culture medium was obtained. Transcriptional profiling of cell lines expressing PGLYRP1 was performed at different stages of C. trachomatis infection. Differential gene expression was studied using the whole transcriptome profiling method on the HumanHT-12 v4 Expression BeadChip microchip using the Illumina Direct Hybridization Whole-Gene Expression Assay protocol. Sample clustering followed by bioinformatics analysis revealed about 100 differentially expressed genes in response to infection with C. trachomatis. PGLYRP1- expressing cells infected with C. trachomatis had a similar transcriptional profile as non-infected cells.
Figure 2. Sample cluster analysis. All data from the two biochips were clustered by the similarity of fluorescence values after sorting the data by the significance level of the detected fluorescence signal (p≤0.05).
Table 1. Scheme of an experiment of transcriptome profiling of cell lines expressing PGLYRP1 after infection with C. trachomatis at different stages of infection.
Table 2. List of common differentially expressed genes in HeLa-PGLYRP1m cell line 1, 24 and 72 hours after infection with C. trachomatis.
Supplementary materials are available at http://dx.doi.org/10.18097/BMCRM00113
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