β1-Adrenergic Receptor Solubilized in the Form of Nanodiscs: Screening of Various Amphipatic Polymers

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Published: Dec 25, 2023
DOI: https://doi.org/10.18097/BMCRM00206
Keywords:
autoantibodies to β1-adrenergic receptor; nanodiscs; amphipatic polymers

Main Article Content

M.M. Peclo
National Medical Research Center of Cardiology named after Academician E.I. Chazov, 15a Academician Chazov str., Moscow, 121552 Russia
L.N. Lipatova
National Medical Research Center of Cardiology named after Academician E.I. Chazov, 15a Academician Chazov str., Moscow, 121552 Russia
N.M. Kashirina
National Medical Research Center of Cardiology named after Academician E.I. Chazov, 15a Academician Chazov str., Moscow, 121552 Russia
T.V. Sharf
National Medical Research Center of Cardiology named after Academician E.I. Chazov, 15a Academician Chazov str., Moscow, 121552 Russia
I.B. Kuznetzova
National Medical Research Center of Cardiology named after Academician E.I. Chazov, 15a Academician Chazov str., Moscow, 121552 Russia
E.E. Efremov
National Medical Research Center of Cardiology named after Academician E.I. Chazov, 15a Academician Chazov str., Moscow, 121552 Russia
E.V. Yanushevskaya
National Medical Research Center of Cardiology named after Academician E.I. Chazov, 15a Academician Chazov str., Moscow, 121552 Russia
P.N. Rutkevich
National Medical Research Center of Cardiology named after Academician E.I. Chazov, 15a Academician Chazov str., Moscow, 121552 Russia
I.N. Rybalkin
National Medical Research Center of Cardiology named after Academician E.I. Chazov, 15a Academician Chazov str., Moscow, 121552 Russia
T.N. Vlasik
National Medical Research Center of Cardiology named after Academician E.I. Chazov, 15a Academician Chazov str., Moscow, 121552 Russia

Abstract

The development of a reliable and easily used diagnostic test for measuring autoantibodies to β1-adrenergic receptor (β1ADR Ab) in patient blood is an unmet clinical need. The enzyme-linked immunosorbent assay (ELISA) is considered as the most appropriate method for this task. In ELISA, the use of peptides corresponding to various fragments of amino acid sequence of β1ADR as antigens leads to inadequate results as β1ADR Ab appear to recognize conformationally dependent epitopes that are generated during the formation of unique tertiary structure of the receptor. Isolation of β1ADR preserving the native conformation and functional characteristics is a quite challenging task. A promising approach to address this task is the use of amphipatic polymers capable of forming nanodiscs, it permits to successfully solubilize membrane proteins. In order to obtain the preparations of solubilized β1ADR that can be used as antigens in ELISA we have tested 17 various amphipatic polymers. The best relative solubilization values (RSV) were obtained using UltrasoluteTM Amphipol 17 (87%) and 18 (62%), as well as by AASTY 11-45 (76%), 11-50 (77%) and 6-50 (78.5%).

Article Details

How to Cite
Peclo, M., Lipatova, L., Kashirina, N., Sharf, T., Kuznetzova, I., Efremov, E., Yanushevskaya, E., Rutkevich, P., Rybalkin, I., & Vlasik, T. (2023). β1-Adrenergic Receptor Solubilized in the Form of Nanodiscs: Screening of Various Amphipatic Polymers. Biomedical Chemistry: Research and Methods, 6(4), e00206. https://doi.org/10.18097/BMCRM00206
Issue
Vol. 6 No. 4 (2023)
Section
EXPERIMENTAL RESEARCH

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