Electrochemical Immunosensor Analysis of Polio Pseudovirus Particles

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Published: Oct 1, 2026
DOI: https://doi.org/10.18097/BMCRM00339
Keywords:
electrochemical immunosensor; poliomyelitis; inactivation; potassium ferricyanide; monoclonal antibodies; gold nanoparticles

Main Article Content

A.V. Berezhnova
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
L.E. Agafonova
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
Yu.Yu. Ivin
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, 8/1 Moskovsky settlement, Polio Institute settlement, Moscow, 108819 Russia
A.N. Pinyaeva
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, 8/1 Moskovsky settlement, Polio Institute settlement, Moscow, 108819 Russia
A.A. Kovpak
Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, 8/1 Moskovsky settlement, Polio Institute settlement, Moscow, 108819 Russia
I.V. Levin
Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, 8/1 Moskovsky settlement, Polio Institute settlement, Moscow, 108819 Russia
A.A. Ishmukhametov
Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, 8/1 Moskovsky settlement, Polio Institute settlement, Moscow, 108819 Russia
S.V. Budnik
CEO Teocortex LLC, 34/6 Pervomaysky settlement, Moscow, 108808 Russia
R.S. Churyukin
CEO Teocortex LLC, 34/6 Pervomaysky settlement, Moscow, 108808 Russia
D.D. Zhdanov
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
A.I. Archakov
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia; Department of Biochemistry, Pirogov Russian National Research Medical University, 1 Ostrovitianova st., Moscow, 117997 Russia
V.V. Shumyantseva
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia; Department of Biochemistry, Pirogov Russian National Research Medical University, 1 Ostrovitianova st., Moscow, 117997 Russia

Abstract

To obtain pseudoviral particles for vaccine production, an electrochemical immunosensor was developed to assess the integrity of the D-antigen of the Sabin strain of poliovirus types 2 and 1 after inactivation. Gold nanoparticles obtained by electrosynthesis on the surface of a working graphite electrode were used to immobilize monoclonal antibodies. The increase of the concentration of the antigen was detected as a decline of the peak current of potassium ferricyanide. The limits of detection (LOD) for the Sabin strains of poliovirus types 1 and 2 were determined to be 9.4 DU/ml and 15 DU/ml, respectively (DU/ml - D-antigen units per milliliter, 1 DU = 15ng). The binding constants (Kb) of the antibody-antigen complexes were calculated and corresponded to 4.68×10⁸ M⁻¹ for Mab2-SII and 1.07×10¹⁰ M⁻¹ for Mab1-SI. High-affinity interactions (Kb ~10⁸ M⁻¹ – 10¹⁰ M⁻¹) in the antibody-antigen systems indicate the preservation of the D-antigen structure after poliovirus inactivation by means of different methods.

Article Details

How to Cite
Berezhnova, A., Agafonova, L., Ivin, Y., Pinyaeva, A., Kovpak, A., Levin, I., Ishmukhametov, A., Budnik, S., Churyukin, R., Zhdanov, D., Archakov, A., & Shumyantseva, V. (2026). Electrochemical Immunosensor Analysis of Polio Pseudovirus Particles. Biomedical Chemistry: Research and Methods, 9(3), e00339. https://doi.org/10.18097/BMCRM00339
Issue
Vol. 9 No. 3 (2026)
Section
EXPERIMENTAL RESEARCH

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