Transcriptional Analysis of HELA Cells - Producers of the Recombinant Peptidoglycan Recognition Protein PGLYRP1 at Different Stages of the Chlamydia Trachomatis Infection Development

  • P.A. Bobrovsky Federal Research and Clinical Center of Physical-Chemical Medicine, 1a Malaya Pirogovskaya str., Moscow, 119435 Russia
  • A.K. Larin Federal Research and Clinical Center of Physical-Chemical Medicine, 1a Malaya Pirogovskaya str., Moscow, 119435 Russia
  • N.F. Polina Federal Research and Clinical Center of Physical-Chemical Medicine, 1a Malaya Pirogovskaya str., Moscow, 119435 Russia
  • V.N. Lazarev Federal Research and Clinical Center of Physical-Chemical Medicine, 1a Malaya Pirogovskaya str., Moscow, 119435 Russia
Keywords: Chlamydia trachomatis; PGLYRP; recombinant proteins; transcriptomics

Abstract

Human peptidoglycan recognition proteins (PGLYRPs) are the components of innate immunity that exhibit antibacterial activity. In this study a cell line secreting recombinant PGLYRP1 into a culture medium was obtained. Transcriptional profiling of cell lines expressing PGLYRP1 was performed at different stages of C. trachomatis infection. Differential gene expression was studied using the whole transcriptome profiling method on the HumanHT-12 v4 Expression BeadChip microchip using the Illumina Direct Hybridization Whole-Gene Expression Assay protocol. Sample clustering followed by bioinformatics analysis revealed about 100 differentially expressed genes in response to infection with C. trachomatis. PGLYRP1- expressing cells infected with C. trachomatis had a similar transcriptional profile as non-infected cells.

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Published
2019-12-23
Section
EXPERIMENTAL RESEARCH