Identification of genes whose mRNAs are subjected to alternative splicing by endonuclease EndoG action in human and murine CD4+ T lymphocytes

Authors

  • D.D. Zhdanov Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia; Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya str., Moscow, 117198 Russia
  • N.S. Novachly Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya str., Moscow, 117198 Russia
  • M.V. Pokrovskaya Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
  • S.S. Aleksandrova Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia
  • T.A. Kabardokov Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya str., Moscow, 117198 Russia
  • N.N. Sokolov Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia

DOI:

https://doi.org/10.18097/BMCRM00128

Keywords:

lymphocytes; EndoG; alternative splicing; sequencing

Abstract

The aim of this work was to identify genes whose mRNAs were subjected to alternative splicing by apoptotic endonuclease EndoG in CD4+ Т lymphocytes from healthy humans, mice, and rats. In order to induce EndoG, lymphocytes were transfected with an EndoG-containing plasmid, or a control pGFP plasmid, or were incubated with cisplatin. Efficiency of transfection, number of cells with DNA damages and the level of EndoG expression have been monitored. Total cell mRNA has been sequenced and the changes in proportion of splice variants of genes were analyzed. The changes in the proportion of 28 mRNA splice variants have been identified in human and murine lymphocytes in both transfected with EndoG gene or incubated with cisplatin. Thus, EndoG can be considered as a potent modulator of alternative splicing of mRNA of identified genes.

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Published

2020-06-30

How to Cite

Zhdanov, D., Novachly, N., Pokrovskaya, M., Aleksandrova, S., Kabardokov, T., & Sokolov, N. (2020). Identification of genes whose mRNAs are subjected to alternative splicing by endonuclease EndoG action in human and murine CD4+ T lymphocytes. Biomedical Chemistry: Research and Methods, 3(2), e00128. https://doi.org/10.18097/BMCRM00128

Issue

Section

EXPERIMENTAL RESEARCH