Sensitivity of Extracellular Vesicles from Human Blood Serum to Various Detergents

Authors

  • A.A. Yakovlev Institute of Higher Nervous Activity and Neurophysiology RAS, 5A Butlerova str., Moscow, 117485 Russia; Scientific and Practical Psychoneurological Center named after Z.P. Solovy’ov DZM, 43 Donskaya str., Moscow, 115419 Russia
  • T.A. Druzhkova Scientific and Practical Psychoneurological Center named after Z.P. Solovy’ov DZM, 43 Donskaya str., Moscow, 115419 Russia
  • A.B. Guekht Scientific and Practical Psychoneurological Center named after Z.P. Solovy’ov DZM, 43 Donskaya str., Moscow, 115419 Russia
  • N.V. Gulyaeva Institute of Higher Nervous Activity and Neurophysiology RAS, 5A Butlerova str., Moscow, 117485 Russia; Scientific and Practical Psychoneurological Center named after Z.P. Solovy’ov DZM, 43 Donskaya str., Moscow, 115419 Russia

DOI:

https://doi.org/10.18097/BMCRM00143

Keywords:

detergents; dynamic light scattering; blood serum; microvesicles; exosomes

Abstract

Blood exosomes and microvesicles, collectively known as small extracellular vesicles (sEV), are vesicles about 100-150 nm in size. Small EV are involved in various aspects of signaling in the body; in addition, they can serve as markers of various pathologies. For biochemical studies, vesicle solubilization is often required. We tested the ability of various detergents to dissolve membranes of the sEV. Small EV were isolated from the blood serum of healthy volunteers by gel filtration on Sepharose CL-2B and tried to solubilize them using the anionic detergent DOC (sodium deoxycholate), non-ionic detergent Brij 35 (polyoxyethyleneglycol dodecyl ether), zwitterionic detergent CHAPS (3 - [(3-chloramidopropyl) dimethylammonio] -1-propanesulfonate), and cationic detergent CTAB (cetyl trimethylammonium bromide). The concentration of sEV in the solution was determined by dynamic light scattering. We find DOC is the most effective for sEV solubilization.

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Published

2020-12-25

How to Cite

Yakovlev, A., Druzhkova, T., Guekht, A., & Gulyaeva, N. (2020). Sensitivity of Extracellular Vesicles from Human Blood Serum to Various Detergents. Biomedical Chemistry: Research and Methods, 3(4), e00143. https://doi.org/10.18097/BMCRM00143

Issue

Section

EXPERIMENTAL RESEARCH