Heterologous Expression of Recombinant L-asparaginase Genes

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Published: Dec 5, 2025
DOI: https://doi.org/10.18097/BMCRM00265
Keywords:
L-asparaginase; expression optimization; heterologous expression; rational and computer-aided design; recombinant genes

Main Article Content

M.V. Pokrovskaya
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121
S.S. Alexandrova
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121
V.S. Pokrovsky
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121
N.D. Dobryakova
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121
A.N. Shishparenok
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121
Yu.V. Gladilina
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121
D.D. Zhdanov
Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121

Abstract

L-asparaginase (EC 3.5.1.1.) is the enzyme with the highest level of global production and is used in the treatment of cancer and in the food industry. Different expression systems are used for the production of many target proteins, ranging from cell-free to hyperproductive plant, insect, bacterial and mammalian cells. This review attempts to bring together the available literature data on heterologous gene expression and technology for the production of recombinant L-asparaginases.

Article Details

How to Cite
Pokrovskaya, M., Alexandrova, S., Pokrovsky, V., Dobryakova, N., Shishparenok, A., Gladilina, Y., & Zhdanov, D. (2025). Heterologous Expression of Recombinant L-asparaginase Genes. Biomedical Chemistry: Research and Methods, 8(4), e00265. https://doi.org/10.18097/BMCRM00265
Issue
Vol. 8 No. 4 (2025): Online first
Section
REVIEWS

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