Сравнительный анализ биоэлектрокаталитических систем, содержащих цитохром Р450 3А4

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П.И. Королева
В.В. Шумянцева

Аннотация

Описаны разработанные авторами подходы для повышения эффективности электроферментативных реакций, катализируемых цитохромом Р450 3А4. Проведен сравнительный анализ цитохром Р450 3А4-систем (i) при образовании функциональных комплексов гемопротеин-флавиновые нуклеотиды как низкомолекулярные модели NADPH-зависимой цитохром Р450 редуктазы, (ii) при образовании продуктивного фермент-субстратного комплекса до стадии получения электронов, поступающих с электрода, (iii) при включении фермента в нанопоры различной природы на электроде (2D-3D переход). Рассмотрены результаты по электрохимическому восстановлению бактосом как функционально активных моделей микросомальной монооксигеназной системы. Для сравнения результатов, полученных для разных моделей, были исследованы электрохимические и электрокаталитические параметры цитохрома Р450 3A4 и маркерного субстрата эритромицина.

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Как цитировать
Королева P., & Шумянцева V. (2024). Сравнительный анализ биоэлектрокаталитических систем, содержащих цитохром Р450 3А4. Biomedical Chemistry: Research and Methods, 7(1), e00210. https://doi.org/10.18097/BMCRM00210
Раздел
ПРОТОКОЛЫ ЭКСПЕРИМЕНТОВ, ПОЛЕЗНЫЕ МОДЕЛИ, ПРОГРАММЫ И СЕРВИСЫ

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